NUCLEOLAR ORGANIZER AND SEX CHROMOSOMES: IS THERE A CORRELATION BETWEEN THEM?
(BY THE EXAMPLE OF ANURA) 

Skorinov Dmitriy Vladimirovich, Candidate of biological sciences, researcher, laboratory of chromosome stability and cell engineering, Institute of Cytology of RAS (4 Tikhoretskiy avenue, Saint-Petersburg, Russia), E-mail: skorinovd@yandex.ru
Pasynkova Roza Abramovna, Junior researcher, laboratory of chromosome stability and cell engineering, Institute of Cytology of RAS (4 Tikhoretskiy avenue, Saint-Petersburg, Russia), E-mail: lilitsraven@yandex.ru
Litvinchuk Spartak Nikolaevich, Candidate of biological sciences, senior researcher, laboratory of chromosome stability and cell engineering, Institute of Cytology of RAS (4 Tikhoretskiy avenue, Saint-Petersburg, Russia), E-mail: litvinchukspartak@yandex.ru 

597.6/.9: 576.316.23 

DOI

10.21685/2307-9150-2019-2-16 

Background. The aim of the work was to study the heteromorphism of nucleolar organizers (NORs) and its correlations with sex chromosomes in different species of Anura.
Materials and methods. NORs in karyotypes of 57 species of tailless amphibians (6 families, 14 genera) were studied. In karyotypes, we analyzed a number of NORs on each chromosome and their total number in the diploid set of chromosomes, size, shape and position of NORs on chromosomes.
Results. Heteromorphy in size and form of NORs was revealed in all studied species. Among species, which were represented in our study by both sexes, 70 % (14 species) had heteromorphic NORs which were unrelated to sex. In the remaining 30 %, only males or only females had heteromorphic NORs. As a rule, NORs were found in a single pair of homologous chromosomes. Additional NORs were extremely rare.
Conclusions. Heteromorphic NOR-bearing chromosomes can be autosomes or sexual ones. 

Key words

Amphibia, nucleolar organizer, sex chromosomes 

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1. Schmid M., Steinlein C., Bogart J. P., Feichtinger W., León P., La Marca E., Díaz L. M., Sanz A., Chen S.-H., Hedges S. B. Cytogenetic and Genome Research. 2010, vol. 130–131, pp. 1–568.
2. Ward O. G. Canadian Journal of Genetics and Cytology. 1977, vol. 19, pp. 51–57.
3. King M. Chromosoma. 1980, vol. 80, pp. 191–217.
4. Foresti F., Toledo L. F. A., Toledo F. S. A. Cytogenetics and Cell Genetics. 1981, vol. 31, pp. 137–144.
5. Schmid M. Chromosoma. 1982, vol. 87, pp. 327–344.
6. Derjusheva S. E., Loginova J. A., Parada R., Chiryaeva O. G., Smirnov A. F., Jaszczak K. Caryologia. 1998, vol. 51 (1), pp. 1–11.
7. Kasahara S., Silva A. P. Z., Gruber S. L., Haddad C. F. B. Cytogenetics and Genome Research. 2003, vol. 103 (1-2), pp. 155–162.
8. Tanomtong A., Khunsook S., Supanuam P., Kaewsri S., Srisamoot N. Cytologia. 2010, vol. 75 (1), pp. 15–21.
9. Danielak-Czech B., Babicz M., Kozubska-Sobocinska A., Rejduch B. Annales Universitatis Mariae Curie-Skłodowska, Sectio E. E.: Zootechnica. 2013, vol. 31 (4), pp. 8–13.
10. Zaleśna A., Florek M., Rybacki M., Ogielska M. Comparative Cytogenetics. 2017, vol. 11 (2), pp. 249–266.
11. Yosida T. R. Journal of Faculty of Science Hokkaido University. Ser. VI, Zoology. 1957, vol. 13, pp. 352–358.
12. Mahony M. J. Genome. 1991, vol. 34, pp. 334–337.
13. Al-Shehri A. H., Al-Saleh A. A. The Journal of Biological Sciences. 2005, vol. 5, pp. 768–770.
14. Skorinov D. V., Bolshakova D. S., Donaire D., Pasynkova R. A., Litvinchuk S. N. Russian Journal of Herpetology. 2018, vol. 25 (4), pp. 253–258.
15. Abramyan, J., Ezaz T., Graves J. A. M., Koopmanand P. Chromosome Research. 2009, vol. 17 (8), pp. 1015–1024.
16. Sessions S. K. Molecular Systematics. Sunderland: Sinauer, 1996, pp. 121–168.
17. Schmid M. Chromosoma. 1978, vol. 66, pp. 345–349.
18. Pisanets E. M., Litvinchuk S. N., Rozanov Yu. M., Reminnyy V. Yu., Pasynkova R. A., Suryadnaya N. N., Matveev A. S. Zbіrnik prats' Zoologіchnogo muzeyu [Proceedings of the Zoological Museum]. 2009, vol. 40 (2008–2009), pp. 83–125.
19. Anderson K. Amphibian Cytogenetics and Evolution. San Diego, 1991, pp. 299–331.
20. Lourenço L. B., Recco-Pimentel S. M., Cardoso A. J. Chromosome Research. 1998, vol. 6, pp. 621–628.
21. Quinderé Y. R. S. D., Lourenço L. B., Andrade G. V., Tomatis C., Baldo D., Recco-Pimentel S. M. Biological Research. 2009, vol. 42, pp. 79–92.
22. Hillis D. M., Green D. M. Journal of Evolutionary Biology. 1990, vol. 3, pp. 49–64.
23. Ezaz T., Stiglec R., Veyrunes F., Graves M. J. Current Biology. 2006, vol. 16, pp. 736–743.
24. Malcom J. W., Kudra R. S., Malone J. H. Journal of Genomics. 2014, vol. 2, pp. 68–76.
25. Perrin P. Evolution. 2009, vol. 63 (12), pp. 3043–3049.
26. Blaser O., Neuenschwander S., Perrin N. The American Naturalist. 2014, vol. 183, pp. 140–146.
27. Dufresnes C., Borzée A., Horn A., Stöck M., Ostini M., Sermier R., Wassef J., Litvinchuck S. N., Kosch T. A., Waldman B., Jang Y., Brelsford A., Perrin N. Molecular Biology and Evolution. 2015, vol. 32 (9), pp. 2328–2337.
28. Frost D. R. Amphibian Species of the World. 2018. Available at: http://research.amnh.org/herpetology/amphibia/index.html (accessed Dec. 11, 2018).
29. Banerjee S. N., Chakrabarti S. Hamadryad. 2003, vol. 27 (2), pp. 248–253.
30. Yang Y. Herpetological Research. 2004, vol. 10, pp. 298–305.
31. Patawang I., Tanomtong A., Phimphan S., Chuaynkern Y., Chuaynkern C., Phaengphairee P., Khrueanet W., Nithikulworawong N. Cytologia. 2014, vol. 79 (2), pp. 141–150.
32. Roco A. S., Olmstead A. W., Degitz S. J., Amano T., Zimmerman L. B., Bullejos M. Proceedings of the National Academy of Sciences of the United States of America. 2015, vol. 112, pp. 4752–4761.
33. Gazoni T., Gruber S. L., Silva A. P. Z., Araújo O. G. S., Narimatsu H., Strüssmann C., Haddad C. F. B., Kasahara S. Chromosoma. 2018, vol. 127 (8), pp. 1–10.
34. Green D. M. Chromosoma. 1988, vol. 97, pp. 55–77.
35. Wiley J. E. Cytogenetic and Genome Research. 2003, vol. 101, pp. 80–83.
36. Odierna G., Aprea G., Capriglione T., Castellano S., Balletto E. Journal of Biosciences. 2007, vol. 32, pp. 763–768.
37. Miura I. Sexual Development. 2007, vol. 1 (6), pp. 323–331.
38. Ogata M., Hasegawa Y., Ohtani H., Mineyama M., Miura I. Heredity. 2008, vol. 100, pp. 92–99.
39. Miura I., Ohtani H., Ogata M. Chromosome Research. 2012, vol. 20, pp. 47–55.
40. Harms J. W. Zoologischer Anzeiger [Zoological bulletin]. 1926, vol. 67, pp. 67–79.
41. Ponse K. La differenciation du sexe et l’intersexualite chez les Vertebres [Differentiation of sex and intersexuality in Vertebrae]. Published by F. Rouge & Sons, 1949, 169 p.
42. Morescalchi A. Bollettino di Zoologia [Bulletin zoology]. 1964, vol. 31, pp. 827–835.
43. Sumida M., Nishioka M. Comparative Biochemistry and Physiology. 2000, vol. 126, pp. 257–270.
44. Jeffries D. L., Lavanchy G., Sermier R., Sredl M. J., Miura I., Borzée A., Barrow L. N., Canestrelli D., Crochet P. A., Dufresnes C., Fu J., Ma W. J., Garcia C. M., Ghali K., Nicieza A. G., O’Donnell R. P., Rodrigues N., Romano A., Martínez-Solano Í., Stepanyan I., Zumbach S., Brelsford A., Perrin N. Nature Communications. 2018, vol. 9, p. 4088.
45. Targueta C. P., Vittorazzi S. E., Gatto K. P., Bruschi D. P., Veiga-Menoncello A. C. P., Pimentel R. S. M., Lourenço L. B. An Essential Guide to Cytogenetics. 2018, 165 p.
46. Charlesworth B., Langley C. H., Stephan W. Genetics. 1986, vol. 112 (4), pp. 947–962.